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Isolation of DNA from Selected Plant Tissues Using Phenol-Chloroform Method
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Olufunke Dawodu *1   , Quawiy Erinle |
| 1- , dawgrace@yahoo.com |
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Abstract: (299 Views) |
DNA extraction is a fundamental technique in molecular biology that involves isolating DNA from biological samples for various downstream applications, including polymerase chain reaction (PCR) and sequencing. This study aimed to extract DNA from plant tissues using the phenol-chloroform method and to compare the responses of different plant leaves to this method. We provide a step-by-step guide for DNA extraction from six different plant speciesFicus capensis (Cape fig or Cape banyan), Ficus exaspirata (Forest Sandpaper), Mangifera indica (Mango), Gmelina arborea (Gamhar), and Bauhinia purpurea (Butterfly tree). The DNA isolation protocol includes several steps: grinding the plant tissue, adding NaCl solution, using a chloroform-isoamyl alcohol mixture to extract DNA, and further purification with phenol-chloroform-isoamyl alcohol. Isopropanol was used to precipitate DNA, and ethanol was used to wash the DNA pellet to remove any remaining contaminants. Finally, the DNA pellet was dissolved in TE buffer for storage. The results demonstrated the successful implementation of the phenol-chloroform method for isolating DNA from selected plant tissues. This study provides a comprehensive protocol for DNA extraction from plant tissues using readily available laboratory reagents and equipment. The protocol can be modified to accommodate different plant species and sample types, facilitating further research in plant genetics and biotechnology. |
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| Keywords: DNA isolation, Plant tissue, Phenol-chloroform method, Centrifugation |
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Type of Study: Original Article |
Subject:
Molecular & Cellolar
Received: 2024/05/31 | Accepted: 2024/09/03 | Published: 2024/10/15
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