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Effect of Ethanolic Extract of Annona Muricata Leaves on Inflammation, Seizure and Liver Enzymes Following Isoniazid Toxicity in Male Wistar Rats
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Emmanuel Ezeokafor *1   , Roy Uchefuna2 , Ikechukwu Emmanuel2 , Doris Ogbuokiri2 , Precious Ebisintei2 , Angela Enendu2 , Obed Aralu2 , Peter Okoye2 , Chidinma Okafor2 |
1- Nnamdi Azikiwe University & None , e.ezeokafor@unizik.edu.ng
2- Nnamdi Azikiwe University & None |
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Abstract: (262 Views) |
Isoniazid is an antibacterial agent primarily used for the treatment of tuberculosis. It functions by inhibiting bacterial growth. Inflammation is a complex defense mechanism of the body that occurs in response to trauma, heat, chemicals, bacteria, and viruses. Annona muricata, commonly known as soursop, is a medicinal plant recognized for its antibacterial, antioxidant, and anti-inflammatory properties. This study aimed to investigate the effects of Annona muricata on inflammation and toxicity following Isoniazid administration. Forty-four male Wistar rats were utilized in this study. Two hundred fifty grams of dried Annona muricata leaves were macerated in 1000 mL of 95% absolute ethanol for 48 hours and administered orally at doses of 100 mg/kg, 500 mg/kg, and 1000 mg/kg. Inflammation was induced by injecting 0.5 mL of egg white into the hind paws, with observations made at 30-minute intervals. Isoniazid (150 mg/kg) was administered intraperitoneally 30 minutes after extract administration, and the rats were observed for two hours for convulsions and mortality. Data were analyzed using ANOVA followed by post hoc Fisher’s LSD, with significance set at p < 0.05. The findings revealed a significant decrease in AST levels, liver weight, seizure frequency, and itching levels. Additionally, there was a significant reduction in paw sizes at 60-120 minutes post-treatment. However, further experiments are warranted to confirm these findings and elucidate the mechanisms underlying the observed decreases in seizure and itching levels. |
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| Keywords: Isoniazid, Annona muricata |
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Type of Study: Original Article |
Subject:
Clinical Medicine
Received: 2024/06/14 | Accepted: 2024/09/25 | Published: 2024/10/17
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