[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 4، شماره 2 - ( 12-1396 ) ::
جلد 4 شماره 2 صفحات 0-0 برگشت به فهرست نسخه ها
In silico Studies for Comparison of FliCs from Salmonella typhimorium, Pseudomonas aeruginosa, and Escherichia coli and their Implication as an Adjuvant
چکیده:   (1195 مشاهده)
An increasing number of studies have demonstrated the effectiveness of flagellin (FliC) as an adjuvant. In this study, FliC(s) from Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Escherichia coli were compared. In silico studies were designed to determine physicochemical parameters of FliC from different bacteria. The fliC gene corresponding to best model was amplified, cloned and expressed into pET28a vector. Bioactivity of the protein was determined by measurement of the interleukin 8 in cell culture after interaction of FliC with TLR-5. According to in silico studies, FliC of S. typhimorium has a higher quality of physiochemical properties, and more affinity to TLR-5 than the other FliCs. SDS-PAGE showed an approximately 54 kDa band, and Western blot confirmed the presence of recombinant protein. IL-8 was expressed significantly in cell culture under S. typhimorium FliC induction. Considering side effects, and costs of conventional adjuvants, FliC can be introduced as a potent natural adjuvant in future studies. In silico studies can save time and cost, and screen potent proteins.
     
نوع مطالعه: Original Article | موضوع مقاله: Medical Biotechnology
دریافت: ۱۳۹۶/۷/۳۰ | پذیرش: ۱۳۹۶/۸/۳۰ | انتشار: ۱۳۹۶/۹/۲۶
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bameri Z, Asadi Karam M R, Habibi M, Oloomi M, Ehsani P, Bouzari S. In silico Studies for Comparison of FliCs from Salmonella typhimorium, Pseudomonas aeruginosa, and Escherichia coli and their Implication as an Adjuvant. IBBJ. 2018; 4 (2)
URL: http://ibbj.org/article-1-158-fa.html

In silico Studies for Comparison of FliCs from Salmonella typhimorium, Pseudomonas aeruginosa, and Escherichia coli and their Implication as an Adjuvant. . 1396; 4 (2)

URL: http://ibbj.org/article-1-158-fa.html



دوره 4، شماره 2 - ( 12-1396 ) برگشت به فهرست نسخه ها
International Biological and Biomedical Journal
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3742