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Application and Usefulness of FISH (Fluorescent In Situ Hybridization) Method in Cytogenetic Researches
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Nevenka Velickova *1   , Azra Drpljanin , Sefedin Biljali |
| 1- , nevenka.velickova@ugd.edu.mk |
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Abstract: (1011 Views) |
Fluorescent in situ hybridization (FISH) is a sensitive molecular cytogenetic method which points to a specific numerical or structural chromosomal aberration. FISH method is a mix of procedure between conventional cytogenetics and molecular genetics. The advantages of this method are the ability to detect genetic aberration in cells in interphase, application on both, fresh and previously fixed samples and short duration of analysis. The sensitivity of this method is great as a possibility of analysis on a large number of cells. The method has a wide range of applications in cytogenetic laboratories for routine analysis in prenatal diagnostics, pediatrics, hematology, oncology and another fields of medicine. FISH method is very important and useful method for quick diagnosis, early and on time prognosis of the disease and initiation of adequate therapy.
Fluorescent in situ hybridization (FISH) is a sensitive molecular cytogenetic method which points to a specific numerical or structural chromosomal aberration. FISH method is a mix of procedure between conventional cytogenetics and molecular genetics. The advantages of this method are the ability to detect genetic aberration in cells in interphase, application on both, fresh and previously fixed samples and short duration of analysis. The sensitivity of this method is great as a possibility of analysis on a large number of cells. The method has a wide range of applications in cytogenetic laboratories for routine analysis in prenatal diagnostics, pediatrics, hematology, oncology and another fields of medicine. FISH method is very important and useful method for quick diagnosis, early and on time prognosis of the disease and initiation of adequate therapy.
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| Keywords: FISH method, Cytogenetic study, application, advantages, sensitivity |
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Type of Study: Letter to the editor |
Subject:
Genetics & Disease
Received: 2022/11/07 | Accepted: 2023/07/13 | Published: 2024/02/27
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