en Clinical Biochemistry Clinical Biochemistry Original Article Original Article <div style="text-align: justify;">Earthworms possess antioxidant, antibacterial, antitumor, and hemolytic properties. To recognize the molecules responsible for various biological activities of earthworm&rsquo;s coelomic fluid, a detailed knowledge about its protein contents is required. The aim of this study was to characterize the proteins present within the coelomic fluid of Eisenia foetida earthworm. Polyacrylamide-gel-electrophoresis (SDS-PAGE), and two-dimensional gel electrophoresis (2-DE) were carried out. Several proteins with molecular masses varying from 10 to 150 kDa were further separated from the coelomic fluid of Eisenia Foetida. The biological activities of the coelomic fluid could be mediated by these proteins.</div> Eisenia foetida, coelomic fluid, electrophoresis 112 116 http://ibbj.org/browse.php?a_code=A-10-86-2&slc_lang=en&sid=1 Golnaz Goodarzi golnaz8920@ymail.com 10031947532846003246 10031947532846003246 No Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran. Durdi Qujeq dqujeq@gmail.com 10031947532846003247 10031947532846003247 Yes Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran. Maryam Mitra Elmi Melmi@yahoo.com 10031947532846003248 10031947532846003248 No Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Farideh Feizi Farideh.fizi@yahoo.com 10031947532846003249 10031947532846003249 No Department of Anatomical Sciences, Babol University of Medical Sciences, Babol, Iran. Sadegh Fattahi S.fattahii@yahho.com 10031947532846003250 10031947532846003250 No Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Nargess Mosavi N.Mosavi@yahoo.com 10031947532846003251 10031947532846003251 No Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran.