en Cell Biology Cell Biology Original Article Original Article <div style="text-align: justify;">In vitro maturation is a highly effective method for achieving mature. Oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. Immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of PMSG. The collected oocytes were divided into two groups: 1.The control group including the base medium 2. The experimental group including base medium supplemented with 25% human follicular fluid.100 oocytes (50 GV oocytes and 50 MI oocytes) were considered for each group. The oocytes of each group were cultured in incubator with 5% CO2 at 37 &ordm;C for 24 hours. Although the resumption of meiosis in the experimental group was more than the control group (44 and 33%, respectively), the difference was not statistically significant (PV>0.05). Moreover, among immature oocytes, the development of GV oocytes to mature oocyte was more than MI oocytes. 50%, 38% of GV oocytes and 28%, 38% of MI oocytes, respectively in the experimental and control groups reached to mature oocyte. Results of the present study demonstrated that a higher percentage of immature oocytes in the experimental group matured in to MII oocytes. It seems that adding follicular fluid to the culture media has positive effects on oocyte maturation. Moreover, GV oocytes are more capable to reach in MII stage compared with MI oocytes in vitro.</div> In vitro maturation, follicular fluid, Germinal Vesicle 0 0 http://ibbj.org/browse.php?a_code=A-10-341-1&slc_lang=en&sid=1 Seyede Zahra Talebian Moghadam 10031947532846004094 10031947532846004094 No Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Seyed Gholam Ali Jorsaraei alijorsara@yahoo.com 10031947532846004095 10031947532846004095 No Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran Ramzan Khanbabaei 10031947532846004096 10031947532846004096 No Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Maryam Gholamitabar Tabari 10031947532846004097 10031947532846004097 No Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran Ali Asghar Ahmadi 10031947532846004098 10031947532846004098 Yes North Research Center, Pasteur Institute of Iran, Amol, Iran