en Clinical Biochemistry Clinical Biochemistry Original Article Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" times="">Background and aims:</span></span></b><span style="line-height:150%"><span new="" roman="" times=""> In recent years due to the daily usage of large amounts of chemical drugs, more attention has been paid to the effect of plants on the treatment and prevention of poisoning caused by these drugs. Herbaceous Ferula assafoetida has antioxidant and anti-inflammatory properties. In this study, the protective effect of Ferula assafoetida hydroalcoholic extract (Fer) on acetaminophen-induced liver toxicity was investigated. </span></span></span></span><br> <span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" times="">Materials and methods:</span></span><strong> </strong></b><span style="line-height:150%"><span new="" roman="" times="">42 mice weighing 25-30 g were randomly divided into six groups. The control group received normal saline and the treatment group gavaged Fer every 12 hours for 7 days 50, 100, 200mg/kg BW and a single dose of acetaminophen (Ace) 500 mg/kg on the 6^th day. One other group received only Ace at a dose of 500 mg/kg BW as intraperitoneally (IP) on the 6^th day, and the last group received only Fer (250mg/kg) orally for one week every 12 hours. At the end of the study (8^th day), Blood was collected by heart puncture, and serum was separated to measure biochemical indices and liver tissues fixed in 10% formalin buffer. One-way ANOVA and Tukey&#39;s analysis at the significance level of P<0.05 were used to analyze data. </span></span></span></span><br> <span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" times="">Results</span></span></b><span style="line-height:150%"><span new="" roman="" times="">: Fer significantly decreased AST, ALT, ALP, T. bilirubin parameters and significantly increased Alb in experimental groups compared to the acetaminophen group.&nbsp; </span></span></span></span><br> <span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" times="">Conclusion</span></span></b><span style="line-height:150%"><span new="" roman="" times="">: According to the results of this study, Fer has a protective role in hepatotoxicity caused by acetaminophen. </span></span></span></span></span></div> Ferula assafoetida, Acetaminophen, Liver poisoning 0 0 http://ibbj.org/browse.php?a_code=A-10-386-1&slc_lang=en&sid=1 Elyas Khayatzadeh elyas.khayatzadeh@yahoo.com 10031947532846004010 10031947532846004010 No Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Firouz Faedmaleki faedmalekif@yahoo.com 10031947532846004011 10031947532846004011 Yes Department of Pharmacology, Babol Branch, Islamic Azad University, Babol, Iran