International Biological and Biomedical Journal

en The wound healing effect of fish scales isolated collagen sponge along with Eisenia foetida glycolipoprotein extract (G-90) in foot ulcer of diabetic rat Other Other Original Article Original Article <div style="text-align: justify;">Treatment of diabetic ulcers is associated with a failure in wound healing. While collagen dressing was applied effectively in wound healing, today, advanced dressing designs that can accelerate healing has received attention. Tissue homogenates glycolipoprotein (G-90) contains some growth factors that can participate in healing. The present study examined the wound healing effects of fish scale collagen sponge along with G-90 on sutured incisions in diabetic rats. G-90 was isolated from earthworm (Eisenia foetida). Type 1 collagen was purified from fish scales and lyophilized in sponge form. The sponges were immersed in 10 ng of G- 90. The efficacy of collagen sponge/G-90 was evaluated on sutured incisions in STZ-induced diabetic rats on days 4, 8, 14, and 18 through a morphological and histological approach. The results demonstrated that collagen sponge/G-90 is effective as a wound healing accelerator all days after wounding. Re-epithelialization and angiogenesis were significantly better in the treated group with collagen sponge/G-90 (P≤0.05). Experimental data demonstrated that collagen sponge/G-90 increased fibroblast proliferation, vascularization, collagen synthesis, and collagen fibril diameters. Considering the existence of many growth factors in G-90 and the role of collagen in the extracellular matrix, we conclude that the collagen sponge/G-90 could be a natural mixture for the management of wound healing. Additional studies are proposed to approve it as a wound-healing agent. </div> collagen sponge, G-90, diabetic rat, earthworm, wound healing 0 0 http://ibbj.org/browse.php?a_code=A-10-92-1&slc_lang=en&sid=1 Maryam M. Elmi mitra_elmi2002@yahoo.com 10031947532846004198 10031947532846004198 No Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran Fatemeh Elmi f.elmi@muz.ac.ir 10031947532846004199 10031947532846004199 No Department of Marine Chemistry, Faculty of Marine & oceanic sciences, University of Mazandaran, Babolsar, Iran Chiman Ghaderi chiman.ghaderi9775@gmail.com 10031947532846004200 10031947532846004200 No Department of Radiation Technology, Paramedical Faculty, Babol University of Medical Sciences, Babol, Iran Farideh Feizi feizi.dr@gmail.com 10031947532846004201 10031947532846004201 Yes Department of Anatomical Science, and Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Science, Babol, Iran