en Molecular & Cellolar Molecular & Cellolar Original Article Original Article <span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><span style="font-size:14.0pt"><span style="line-height:150%"><span style="font-family:&quot;Times New Roman&quot;,serif">Colorectal cancer is one of the most common types of cancer and one of the deadliest types of malignancies worldwide. In recent years, extensive studies have been conducted on the effect of medicinal plants and their anti-cancer and anti-tumor effects, of which licorice is one of them. The root and the body of this plant are the two main parts of this plant, which therapeutic properties have been observed. This study was conducted with the aim of investigating the cytotoxic effect of ethanolic extract of licorice root (GE) on HT-29 cell line. HT-29 cells were treated with different concentrations of extract (50, 100, 200, 400, 800, 1600 and 3200 &mu;g/ml). The MTT method was employed to evaluate the cytotoxicity of GE. The results showed that the highest rate of cell death was at the concentration of 447.2 &mu;g/ml. Also, in the GC-MS evaluation of this extract, many effective substances with anticancer properties were observed. In conclusion, GE seemed to have the ability to exert anticancer effects on epithelial colorectal cancer cells.</span></span></span></span></span></span> Apoptosis, Licorice root, Colorectal cancer, HT-29, MTT 0 0 http://ibbj.org/browse.php?a_code=A-10-400-1&slc_lang=en&sid=1 Alireza Salehi Alireza.salehi74@yahoo.com 10031947532846004154 10031947532846004154 Yes Department of Pathology, Islamic Azad University, Babol branch, Iran Atefeh Sadat Nabavi atefehnab@gmail.com 10031947532846004155 10031947532846004155 No Department of Pathology, Islamic Azad University, Babol branch, Iran