International Biological and Biomedical Journal

en Benefits of testing for the G12D mutation of the KRAS gene in hepatocellular carcinoma (HCC) in Senegal Genomics & Proteomics Genomics & Proteomics Original Article Original Article Background: The mutation of the KRAS proto-oncogene is frequently detected in various cancers, including hepatocellular carcinoma (HCC). This mutation often results in the failure of treatments based on tyrosine kinase receptor inhibitors. The study's objective was to investigate mutations of codon 12 and 13 of the KRAS gene and assess the risk of developing HCC. Methods: A prospective, cross-sectional, and analytical study was conducted from November 2018 to October 2021 at the biochemistry and molecular biology laboratory of UCAD's Faculty of Medicine. Mutations of codons 12 and 13 were identified using HRM and confirmed by sequencing. Hepatic assessments (transaminases, GGT, PAL, bilirubin, and AFP) were performed on all patients, and histopathological analysis was conducted. Results: The study consisted of 34 patients with an average age of 44±3.4 years and a sex ratio of 5.8. There was a predominance in the age group of 36 to 45 years (38.2%). The prevalence of the G12D mutation of the KRAS gene was 23.5% and was more common in subjects over 45 years (n=5; 11.7%). Hepatic impairments were less pronounced in carriers of the G12D mutation of the KRAS gene, albeit not significantly different. Additionally, tumors of grade 3 and type pT3 appeared more linked to the G12D mutation of the KRAS gene, with prevalences of 18.75% (n=3) and 30.8% (n=4), respectively. The relationship between chronic HBV carriage and the KRAS gene mutation was not significant. Conclusion: Molecular biology significantly contributes to diagnosis and patient management by identifying molecular markers for pre-therapeutic evaluation.   codon 12 and 13 KRAS, HBV, hepatocellular carcinoma, Senegal. 0 0 http://ibbj.org/browse.php?a_code=A-10-411-1&slc_lang=en&sid=1 dia fatou kine sy thorpe sythorpediaa@gmail.com 10031947532846004174 10031947532846004174 Yes Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal thiam souleymane sthiam85@gmail.com 10031947532846004175 10031947532846004175 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal samba abdourahmane arsamba76@gmail.com 10031947532846004176 10031947532846004176 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal soumah idrissa yaya soumahidi.iys@gmail.com 10031947532846004177 10031947532846004177 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal diedhiou fatou fatoudiedhiou8990@gmail.com 10031947532846004178 10031947532846004178 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal. medece hortence honorine agossou hortenceagossou@gmail.com 10031947532846004179 10031947532846004179 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal. ka ibrahima dribouka@gmail.com 10031947532846004180 10031947532846004180 No Surgical Department, General Hospital of Grand Yoff, Dakar, Senegal deguenovo gabriel deguenovogabriel@yahoo.fr 10031947532846004181 10031947532846004181 No Cytology and Pathology Department, General Hospital of Grand Yoff, Dakar, Senegal cisse fatou kinciss@yahoo.fr 10031947532846004182 10031947532846004182 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Dalal Jamm Hospital, Dakar, Senegal ndiaye arame yssndiaye@yahoo.fr 10031947532846004183 10031947532846004183 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal coly najah fatou najahfatoucoly@gmail.com 10031947532846004184 10031947532846004184 No Pharmaceutical Biochemestry/Department of Medical Biology and Functional Exploration, Faculty of Health, Thies University, Thies, Senegal dial cherif mouhamed dialcherif@yahoo.fr 10031947532846004185 10031947532846004185 No Cytology and Pathology Department, General Hospital of Grand Yoff, Dakar, Senegal Diop pape saloum Dioppapasaloum@yahoo.fr 10031947532846004186 10031947532846004186 No Surgical Department, General Hospital of Grand Yoff, Dakar, Senegal Diallo fatou fadiallo@hotmail.com 10031947532846004187 10031947532846004187 No Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal