International Biological and Biomedical Journal

en Effect of Ethanolic Extract of Annona Muricata Leaves on Inflammation, Seizure and Liver Enzymes Following Isoniazid Toxicity in Male Wistar Rats Clinical Medicine Clinical Medicine Original Article Original Article <div style="text-align: justify;">Isoniazid is an antibacterial agent primarily used for the treatment of tuberculosis. It functions by inhibiting bacterial growth. Inflammation is a complex defense mechanism of the body that occurs in response to trauma, heat, chemicals, bacteria, and viruses. Annona muricata, commonly known as soursop, is a medicinal plant recognized for its antibacterial, antioxidant, and anti-inflammatory properties. This study aimed to investigate the effects of Annona muricata on inflammation and toxicity following Isoniazid administration. Forty-four male Wistar rats were utilized in this study. Two hundred fifty grams of dried Annona muricata leaves were macerated in 1000 mL of 95% absolute ethanol for 48 hours and administered orally at doses of 100 mg/kg, 500 mg/kg, and 1000 mg/kg. Inflammation was induced by injecting 0.5 mL of egg white into the hind paws, with observations made at 30-minute intervals. Isoniazid (150 mg/kg) was administered intraperitoneally 30 minutes after extract administration, and the rats were observed for two hours for convulsions and mortality. Data were analyzed using ANOVA followed by post hoc Fisher’s LSD, with significance set at p < 0.05. The findings revealed a significant decrease in AST levels, liver weight, seizure frequency, and itching levels. Additionally, there was a significant reduction in paw sizes at 60-120 minutes post-treatment. However, further experiments are warranted to confirm these findings and elucidate the mechanisms underlying the observed decreases in seizure and itching levels.</div> Isoniazid, Annona muricata 0 0 http://ibbj.org/browse.php?a_code=A-10-421-1&slc_lang=en&sid=1 Emmanuel Ezeokafor e.ezeokafor@unizik.edu.ng 10031947532846004327 10031947532846004327 Yes Nnamdi Azikiwe University Roy Uchefuna rc.uchefuna@unizik.edu.ng 10031947532846004328 10031947532846004328 No Nnamdi Azikiwe University Ikechukwu Emmanuel monamiokafor@gmail.com 10031947532846004329 10031947532846004329 No Nnamdi Azikiwe University Doris Ogbuokiri kd.Ogbuokiri@unizik.edu.ng 10031947532846004330 10031947532846004330 No Nnamdi Azikiwe University Precious Ebisintei sinteiprecious@gmail.com 10031947532846004331 10031947532846004331 No Nnamdi Azikiwe University Angela Enendu ac.enendu@unizik.edu.ng 10031947532846004332 10031947532846004332 No Nnamdi Azikiwe University Obed Aralu araluobed@gmail.com 10031947532846004333 10031947532846004333 No Nnamdi Azikiwe University Peter Okoye Kelvincastor.okoye@gmail.com 10031947532846004334 10031947532846004334 No Nnamdi Azikiwe University Chidinma Okafor okaforchidinma@gmail.com 10031947532846004335 10031947532846004335 No Nnamdi Azikiwe University