ORIGINAL_ARTICLE When all the bacteria in cavity walls cannot be eliminated during the cleaning of dental caries, the use of cavity disinfectants is neccessary. The aim of this study was to evaluate the effect of different disinfectants on the bonding strength of adhesive materials polymerised without light. A total of 60 3rd molar teeth, extracted for various reasons, were used in the study. The dentin surfaces were exposed by first raising the enamel tissue with a carbon separator. All the teeth were then embedded in acrylic. After preparation, the total 60 samples were separated into 4 groups of 15 for the use of different disinfectant solutions. Group 1 was defined as the control group and no cavity disinfectant was applied. In the first stage of the restoration, Tokuyama Universal Bond was applied. After application of the adhesive, the samples were placed in prepared cylindrical tubes, 2.3mm in diameter and 3mm in height so that the dentin surface was completely central, Estelite posterior quick composite was applied and then polymerisation. In Group 2, the dentin surfaces were first disinfected for 6 secs with ozone gas produced from a Prozone device. In Group 3, the dentin surfaces were cleaned for 60 secs with 2% chlorhexidine gluconate solution, then dried foor 10 secs with light pressure air. In Group 4, 2.5% sodium hypochlorite (NaOCl) was applied to the dentin surfaces as cavity disinfectant. The restoration stages of all the cavities were completed in the same way as for Group 1. The samples with completed restorations were then placed in a instron universal test device to evaluate the bonding strength resistance to shear stress. Statistical evaluation of the results was made using the Kruskal Wallis and the Mann Whitney U-tests. It was seen that ozone (9,04) and chlorhexidine gluconate (6,59) increased the bonding strength of adhesive resins whereas sodium hypochlorite (2,82) reduced it (p<0.05). From the results of this study, it was concluded that chlorhexidine gluconate and especially ozone, can be safely used as cavity disinfectants. http://ibbj.org/article-1-225-en.pdf 2019-08-15 0 0 Ozone non-light curing bond cavity disinfectant Suzan Cangul suzanbali@outlook.com 1 Dicle University Faculty Of Dentistry, Department Of Restorative Dentistry Diyarbakir, TURKEY. AUTHOR Ozkan Adiguzel dentamania21@hotmail.com 2 Dicle University, Faculty Of Dentistry, Department Of Endodontics, Diyarbakir, TURKEY. AUTHOR Server Unal servermutluay@hotmail.com 3 Afyonkarahisar Health Sciences University Faculty Of Dentistry, Department Of Prosthodontics, Afyonkarahisar, TURKEY. AUTHOR Mustafa Orkun Ertugrul orkunertugrul@yandex.com 4 Dicle University Faculty Of Dentistry, Department Of Restorative Dentistry Diyarbakir, TURKEY. AUTHOR Simge Gumus dtsimgegumus@gmail.com 5 Dicle University Faculty Of Dentistry, Department Of Restorative Dentistry Diyarbakir, TURKEY. AUTHOR Samican unal samican1507@hotmail.com 6 Dicle University Faculty Of Dentistry, Department Of Restorative Dentistry Diyarbakir, TURKEY. AUTHOR Begüm Erpaçal begumerpacal@gmail.com 7 Dicle University Faculty Of Dentistry, Department Of Restorative Dentistry Diyarbakir, TURKEY. AUTHOR

ORIGINAL_ARTICLE Background: The mutation of the KRAS proto-oncogene is frequently detected in various cancers, including hepatocellular carcinoma (HCC). This mutation often results in the failure of treatments based on tyrosine kinase receptor inhibitors. The study's objective was to investigate mutations of codon 12 and 13 of the KRAS gene and assess the risk of developing HCC. Methods: A prospective, cross-sectional, and analytical study was conducted from November 2018 to October 2021 at the biochemistry and molecular biology laboratory of UCAD's Faculty of Medicine. Mutations of codons 12 and 13 were identified using HRM and confirmed by sequencing. Hepatic assessments (transaminases, GGT, PAL, bilirubin, and AFP) were performed on all patients, and histopathological analysis was conducted. Results: The study consisted of 34 patients with an average age of 44±3.4 years and a sex ratio of 5.8. There was a predominance in the age group of 36 to 45 years (38.2%). The prevalence of the G12D mutation of the KRAS gene was 23.5% and was more common in subjects over 45 years (n=5; 11.7%). Hepatic impairments were less pronounced in carriers of the G12D mutation of the KRAS gene, albeit not significantly different. Additionally, tumors of grade 3 and type pT3 appeared more linked to the G12D mutation of the KRAS gene, with prevalences of 18.75% (n=3) and 30.8% (n=4), respectively. The relationship between chronic HBV carriage and the KRAS gene mutation was not significant. Conclusion: Molecular biology significantly contributes to diagnosis and patient management by identifying molecular markers for pre-therapeutic evaluation.   http://ibbj.org/article-1-291-en.pdf 2024-02-27 0 0 codon 12 and 13 KRAS HBV hepatocellular carcinoma Senegal. dia fatou kine sy thorpe sythorpediaa@gmail.com 1 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal AUTHOR thiam souleymane sthiam85@gmail.com 2 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal AUTHOR samba abdourahmane arsamba76@gmail.com 3 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal AUTHOR soumah idrissa yaya soumahidi.iys@gmail.com 4 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal AUTHOR diedhiou fatou fatoudiedhiou8990@gmail.com 5 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal. AUTHOR medece hortence honorine agossou hortenceagossou@gmail.com 6 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal. AUTHOR ka ibrahima dribouka@gmail.com 7 Surgical Department, General Hospital of Grand Yoff, Dakar, Senegal AUTHOR deguenovo gabriel deguenovogabriel@yahoo.fr 8 Cytology and Pathology Department, General Hospital of Grand Yoff, Dakar, Senegal AUTHOR cisse fatou kinciss@yahoo.fr 9 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Dalal Jamm Hospital, Dakar, Senegal AUTHOR ndiaye arame yssndiaye@yahoo.fr 10 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal AUTHOR coly najah fatou najahfatoucoly@gmail.com 11 Pharmaceutical Biochemestry/Department of Medical Biology and Functional Exploration, Faculty of Health, Thies University, Thies, Senegal AUTHOR dial cherif mouhamed dialcherif@yahoo.fr 12 Cytology and Pathology Department, General Hospital of Grand Yoff, Dakar, Senegal AUTHOR Diop pape saloum Dioppapasaloum@yahoo.fr 13 Surgical Department, General Hospital of Grand Yoff, Dakar, Senegal AUTHOR Diallo fatou fadiallo@hotmail.com 14 Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Cheikh Anta Diop University, Dakar, Senegal.Laboratory of Biochemistry, Aristide Le Dantec Hospital, Dakar, Senegal AUTHOR