International Biological and Biomedical Journal
IBBJ
Medical Sciences
http://ibbj.org
1
admin
2423-4478
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7
14
2423
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en
jalali
1395
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gregorian
2017
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online
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fulltext
en
Antibacterial Activities of Extracellular Metabolites of Symbiotic Bacteria, Xenorhabdus and Photorhabdus Isolated from Entomopathogenic Nematodes
Medical Biotechnology
Medical Biotechnology
Original Article
Original Article
<p style="text-align: justify;">Xenorhabdus and Photorhabdus are members of the family Enterobacteriaceae, and are engaged in a mutualistic association with the entomopathogenic nematodes, Steinernema and Heterorhabditis, respectively. In the present study, the organic fraction of ethyl acetate bacterial crude extracts of the symbiotic bacteria, Xenorhabdus stockiae and Photorhabdus luminescens isolated from entomopathogenic nematodes, were characterized by High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared Spectroscopic (FTIR) methods, and evaluated for their antibacterial activity on selected pathogenic bacteria, Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumonia by agar well plate method. When compared to X. stockiae, P. luminescens showed higher antibacterial activity at maximum concentration on K. pneumonia and S. aureus, respectively. FTIR analysis of the crude ethyl acetate extract of X. stockiae showed the corresponding peaks values of 2373.33 and 544.93 cm-1 whereas P. luminescens showed the presence of peaks at 2128.00 and 552.80 cm-1, respectively indicating the functional group of P-H and isocyanides stretching. The present study suggests that the antibacterial activity might be due to the effective compounds from the symbiotic bacteria of entomopathogenic nematodes, and that they could be used as bioinsecticides in the future.</p>
Steinernema, Heterorhabditis, Xenorhabdus, Photorhabdus, antimicrobial activity
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http://ibbj.org/browse.php?a_code=A-10-194-1&slc_lang=en&sid=1
Dilipkumar
Aiswarya
aisubit@gmail.com
10031947532846002364
10031947532846002364
No
Ramalingam
Karthik Raja
sriramkarthik83@gmail.com
10031947532846002365
10031947532846002365
No
Govindhan
Gowthaman
gowthambt007@gmail.com
10031947532846002366
10031947532846002366
No
Paramasivam
Deepak
deepaksiva1252@gmail.com
10031947532846002367
10031947532846002367
No
Govindasamy
Balasubramani
balabio62@gmail.com
10031947532846002368
10031947532846002368
No
Pachiappani
Perumal
perumalarticles@gmail.com
10031947532846002369
10031947532846002369
Yes