International Biological and Biomedical Journal
IBBJ
Medical Sciences
http://ibbj.org
1
admin
2423-4478
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7
14
2423
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en
jalali
1394
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1
gregorian
2015
6
1
1
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online
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fulltext
en
The Serotonin 5-HT2A Receptor Antagonist Ritanserin Induces Apoptosis in Human Colorectal Cancer and Acts in Synergy with Curcumin
Cancer
Cancer
Original Article
Original Article
<p class="MsoNormal" dir="ltr" style="line-height: 150% background: rgb(242, 219, 219) none repeat scroll 0% 0% text-align: justify">Curcumin exhibits both cancer- preventive activity and growth inhibitory effects on several neoplastic cells including human colon cancer. Serotonin and its<sub> </sub>receptors have also been implicated in tumor development. This study investigated the effect of ritanserin, a selective serotonin 5HT<sub>2A</sub> receptor antagonist, alone and in combination with curcumin on colorectal cancer cell lines. Results show that the expression of the serotonin 5HT<sub>2A</sub> receptor is higher in tumor than in normal colorectal tissues. Ritanserin, reduced cell viability in a dose-dependent manner, and increased apoptosis in HT29, SW480 and SW742 colorectal cancer cell lines as analyzed by MTT and TUNEL assays, respectively. Moreover, combined with curcumin, ritanserin synergistically increased the number of hypodiploid cells and DNA fragmentation and decreased cell viability in all colorectal cancer cell lines. This study demonstrates that curcumin and ritanserin have a synergic anti- mitogenic and apoptotic effect on colorectal cancer cell lines. These results suggest a potential use of serotonin 5HT<sub>2A </sub>receptor antagonist in co- treatment with curcumin in colon cancer therapy.</p>
Colon cancer, serotonin 5HT2A receptor, cell growth, ritanserin, TUNEL assay
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http://ibbj.org/browse.php?a_code=A-10-33-1&slc_lang=en&sid=1
Ali Asghar
Ahmadi
ahmadi.pasteur@gmail.com
10031947532846001740
10031947532846001740
Yes
Mohammad
Shadifar
10031947532846001741
10031947532846001741
No
Ramin
Ataee
10031947532846001742
10031947532846001742
No
Cathy
Vaillancourt
raminataee1349@gmail.com
10031947532846001743
10031947532846001743
No
Amin
Ataee
10031947532846001744
10031947532846001744
No
Talal
Oufkir
10031947532846001745
10031947532846001745
No
Majid
Jafari-Sabet
10031947532846001746
10031947532846001746
No