[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 3, Issue 2 (Int Biol Biomed J 2017) ::
IBBJ 2017, 3(2): 80-88 Back to browse issues page
Antibacterial Activities of Extracellular Metabolites of Symbiotic Bacteria, Xenorhabdus and Photorhabdus Isolated from Entomopathogenic Nematodes
Dilipkumar Aiswarya , Ramalingam Karthik Raja , Govindhan Gowthaman , Paramasivam Deepak , Govindasamy Balasubramani , Pachiappani Perumal * 1
1- , perumalarticles@gmail.com
Abstract:   (1885 Views)

Xenorhabdus and Photorhabdus are members of the family Enterobacteriaceae, and are engaged in a mutualistic association with the entomopathogenic nematodes, Steinernema and Heterorhabditis, respectively. In the present study, the organic fraction of ethyl acetate bacterial crude extracts of the symbiotic bacteria, Xenorhabdus stockiae and Photorhabdus luminescens isolated from entomopathogenic nematodes, were characterized by High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared Spectroscopic (FTIR) methods, and evaluated for their antibacterial activity on selected pathogenic bacteria, Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumonia by agar well plate method. When compared to X. stockiae, P. luminescens showed higher antibacterial activity at maximum concentration on K. pneumonia and S. aureus, respectively. FTIR analysis of the crude ethyl acetate extract of X. stockiae showed the corresponding peaks values of 2373.33 and 544.93 cm-1 whereas P. luminescens showed the presence of peaks at 2128.00 and 552.80 cm-1, respectively indicating the functional group of P-H and isocyanides stretching. The present study suggests that the antibacterial activity might be due to the effective compounds from the symbiotic bacteria of entomopathogenic nematodes, and that they could be used as bioinsecticides in the future.

Keywords: Steinernema, Heterorhabditis, Xenorhabdus, Photorhabdus, antimicrobial activity
Full-Text [PDF 904 kb]   (598 Downloads)    
Type of Study: Original Article | Subject: Medical Biotechnology
Received: 2017/02/8 | Accepted: 2017/03/20 | Published: 2017/03/29
Add your comments about this article
Your username or Email:

Write the security code in the box >


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aiswarya D, Karthik Raja R, Gowthaman G, Deepak P, Balasubramani G, Perumal P. Antibacterial Activities of Extracellular Metabolites of Symbiotic Bacteria, Xenorhabdus and Photorhabdus Isolated from Entomopathogenic Nematodes. IBBJ. 2017; 3 (2) :80-88
URL: http://ibbj.org/article-1-110-en.html


Volume 3, Issue 2 (Int Biol Biomed J 2017) Back to browse issues page
International Biological and Biomedical Journal
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3658