[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 3, Issue 3 (Int Biol Biomed J 2017) ::
IBBJ 2017, 3(3): 119-124 Back to browse issues page
Hepatotoxicity and ALT/AST Enzymes Activities Change in Therapeutic and Toxic Doses Consumption of Acetaminophen in Rats
Pouria Jarsiah 1, Anahita Nosrati 2, Ahad Alizadeh 3, Seyed Mohammad Bagher Hashemi-Soteh * 4
1- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
2- Laboratory of Pathobiology, Imam Khomeini Hospital, Sari, Iran.
3- Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
4- Immunogenetic Research center, Molecular and Cell biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. , hashemisoteh@gmail.com
Abstract:   (1653 Views)

Acetaminophen (APAP) poisoning is the most common drug intoxication, which often leads to acute liver failure (ALF). In this study, the effects of different doses of APAP on aminotransferases (AST and ALT) and liver pathological lesions were assessed in young rats. 32 male albino Wistar rats were randomly selected and divided into eight groups. In case groups, three groups after one hour, and 3 groups after three hours were sampled and sacrificed after intraperitoneal injection of 70, 150 and 300 mg/Kg.bw APAP. The variances of necropsy and macroscopic features were recorded after fixation in 10% formalin and staining with hematoxylin and eosin. There was no pathological change in the one or three hours at 75 mg/kg. The 150 mg/kg dose caused mild hyperemia and edema of the portal areas and mild infiltration of inflammatory cells at both one hour and three hours time points. Centrilobular necrosis was mild at 300 mg/kg after one and three hours, and hepatocellular necrosis was sporadically and sligthly elavated for this dose after one hour. Based on multivariate variance analysis test (MANOVA), both dose and time exerted significant effects on ALT and AST activities in the case groups at the first and third hours (P < 0.05). Due to the ability to induce a protective system against acetaminophen toxicity, ALT plasma level evaluation in the early hours will be more helpful than measuring AST level.

Keywords: Acetaminophen, drug toxicity, ALT, AST, hepatic necrosis, rat
Full-Text [PDF 812 kb]   (1477 Downloads)    
Type of Study: Original Article | Subject: Histopathology
Received: 2017/05/7 | Accepted: 2017/06/23 | Published: 2017/07/1
Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jarsiah P, Nosrati A, Alizadeh A, Hashemi-Soteh S M B. Hepatotoxicity and ALT/AST Enzymes Activities Change in Therapeutic and Toxic Doses Consumption of Acetaminophen in Rats. IBBJ. 2017; 3 (3) :119-124
URL: http://ibbj.org/article-1-129-en.html


Volume 3, Issue 3 (Int Biol Biomed J 2017) Back to browse issues page
International Biological and Biomedical Journal
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3781