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Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm
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Golnaz Goodarzi1   , Durdi Qujeq *2 , Maryam Mitra Elmi3 , Farideh Feizi4 , Sadegh Fattahi3 , Nargess Mosavi3 |
1- Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran.
2- Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran. , dqujeq@gmail.com
3- Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
4- Department of Anatomical Sciences, Babol University of Medical Sciences, Babol, Iran. |
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Abstract: (8464 Views) |
Earthworms possess antioxidant, antibacterial, antitumor, and hemolytic properties. To recognize the molecules responsible for various biological activities of earthworm’s coelomic fluid, a detailed knowledge about its protein contents is required. The aim of this study was to characterize the proteins present within the coelomic fluid of Eisenia foetida earthworm. Polyacrylamide-gel-electrophoresis (SDS-PAGE), and two-dimensional gel electrophoresis (2-DE) were carried out. Several proteins with molecular masses varying from 10 to 150 kDa were further separated from the coelomic fluid of Eisenia Foetida. The biological activities of the coelomic fluid could be mediated by these proteins. |
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| Keywords: Eisenia foetida, coelomic fluid, electrophoresis |
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Full-Text [PDF 433 kb]
(3073 Downloads)
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Type of Study: Original Article |
Subject:
Clinical Biochemistry
Received: 2017/11/13 | Accepted: 2017/12/01 | Published: 2018/01/01
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