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:: Volume 5, Issue 1 (Int Biol Biomed J (In press) 2019) ::
IBBJ 2019, 5(1): 0-0 Back to browse issues page
Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea
Rakesh Kumar Singh 1, Garima Tripathi , Pawan Kumar Dubey * 2
1- Department of Biotechnology, TNB College, Bhagalpur-812007, India.
2- Center for Genetic Disorders, Institute of Science, Banaras Hindu University, Varanasi-221005, India. , pawandubey1981@gmail.com
Abstract:   (77 Views)
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in both humans and animals. The contaminated food and water are the most common vehicles for ETEC infection. The colonization factor antigen (CFA-1) is a fimbriae protein that promotes adherence of the ETEC strain to the epithelium of the small intestine of the host. In this study IgY proteins were produced against the CFA-1 of ETEC in immunized white leg horn chickens. The collection of antibody was found higher in 25% and 50% ammonium precipitated samples. The isolated CFA-1 protein (0.5 mg/ml) was purified by dialysis (0.42 mg/ml), gel-filtration (0.08 mg/ml), and ion-exchange chromatography (0.065 mg/ml). The CFA-1 protein obtained was submitted to pBLAST which matched with CFA-b with accession no.-AAC41415.1. The CFA-1 gene was cloned in pUC18 vector, and transformed into Escherichia coli DH5α strain. In conclusion, we have successfully purified and characterized monoclonal antibodies (IgY proteins) against CFA-1 of enterotoxigenic E. coli that may be useful for diagnosis of widely occurring Escherichia coli infections.
Keywords: Enterotoxigenic Escherichia coli, diarrhea, colonization factor antigen
     
Type of Study: Original Article | Subject: Infectious Disease
Received: 2019/02/21 | Accepted: 2019/04/6 | Published: 2019/05/12
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Singh R K, Tripathi G, Dubey P K. Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea. IBBJ. 2019; 5 (1)
URL: http://ibbj.org/article-1-215-en.html


Volume 5, Issue 1 (Int Biol Biomed J (In press) 2019) Back to browse issues page
International Biological and Biomedical Journal
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