When all the bacteria in cavity walls cannot be eliminated during the cleaning of dental caries, the use of cavity disinfectants is neccessary. The aim of this study was to evaluate the effect of different disinfectants on the bonding strength of adhesive materials polymerised without light. A total of 60 3rd molar teeth, extracted for various reasons, were used in the study. The dentin surfaces were exposed by first raising the enamel tissue with a carbon separator. All the teeth were then embedded in acrylic. After preparation, the total 60 samples were separated into 4 groups of 15 for the use of different disinfectant solutions. Group 1 was defined as the control group and no cavity disinfectant was applied. In the first stage of the restoration, Tokuyama Universal Bond was applied. After application of the adhesive, the samples were placed in prepared cylindrical tubes, 2.3mm in diameter and 3mm in height so that the dentin surface was completely central, Estelite posterior quick composite was applied and then polymerisation. In Group 2, the dentin surfaces were first disinfected for 6 secs with ozone gas produced from a Prozone device. In Group 3, the dentin surfaces were cleaned for 60 secs with 2% chlorhexidine gluconate solution, then dried foor 10 secs with light pressure air. In Group 4, 2.5% sodium hypochlorite (NaOCl) was applied to the dentin surfaces as cavity disinfectant. The restoration stages of all the cavities were completed in the same way as for Group 1. The samples with completed restorations were then placed in a instron universal test device to evaluate the bonding strength resistance to shear stress. Statistical evaluation of the results was made using the Kruskal Wallis and the Mann Whitney U-tests. It was seen that ozone (9,04) and chlorhexidine gluconate (6,59) increased the bonding strength of adhesive resins whereas sodium hypochlorite (2,82) reduced it (p<0.05). From the results of this study, it was concluded that chlorhexidine gluconate and especially ozone, can be safely used as cavity disinfectants.