Cigarette smoke contains three primary toxins: carbon monoxide (CO), nicotine, and tar, which can lead to an increase in Reactive Oxygen Species (ROS) and a decrease in antioxidant reserves. The depletion of antioxidant reserves can damage testicular cells, inhibiting the division of germinal testicular cells. Consequently, testosterone production is also inhibited, adversely affecting the quality of spermatogenesis. Red dragon fruit juice may serve as a potential antioxidant that mitigates ROS, thereby preventing damage to testicular cells. This research employed a "post-test only control group design." Twenty-five laboratory rats were divided into groups: KP (positive control), KN (negative control), P1, P2, and P3 (exposed to cigarette smoke for 15 minutes and subsequently administered dragon fruit juice at doses of 1.44 cc, 2.88 cc, and 4.32 cc, respectively) for 24 days. On the 32nd day, serum testosterone concentration was determined using ELISA. Additionally, sperm count and morphology were assessed using a digital microscope. Data were analyzed using one-way ANOVA, with a significance level set at p<0.05.The results of this research are summarized as follows: the average serum testosterone concentrations were KN 2.29 ng/ml, KP 2.65 ng/ml, P1 2.35 ng/ml, P2 2.86 ng/ml, and P3 2.39 ng/ml. The sperm counts were as follows: KN 399 million/ml, KP 269 million/ml, P1 312 million/ml, P2 357 million/ml, and P3 362 million/ml. The morphology of spermatozoa was recorded as follows: KN 72.01%, P1 81.09%, P2 82.33%, and P3 87.70%. Statistical analysis using one-way ANOVA indicated that the concentration of serum testosterone was not significantly different (p=0.109), while significant differences were observed in sperm count (p=0.005) and sperm morphology (p=0.005).Based on the statistical analysis, red dragon fruit juice did not significantly affect serum testosterone hormone concentration. However, it did positively influence the quality of spermatozoa in rats exposed to cigarette smoke, resulting in increases in both sperm count and morphology.