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RNA extraction from bone and cartilage of patient with developmental dysplasia of hip type 4: a new simplified process to obtain total RNA.
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Afshin Taheriazam1   , Sana Akrami * 2, Mohsen Yari3 |
1- Department of Orthopedics, Faculty of medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Faculty of medicine, Tabriz Medical Sciences, Islamic Azad University: Tabriz, Tabriz, IR , sana363a@yahoo.com
3- Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, P. O. Box 14115-154, Tehran, Iran, |
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Abstract: (492 Views) |
Considering the difficulty of RNA extraction from tissues such as bone and cartilage in human, mainly due to their unworkable nature, few research have been conducted in this area and it had always appeared as a challenge to scientist. Although recently more and more approach has been directed toward this path, considering the medical, preventive, hereditary and therapeutical importance of RNA extraction to be then used by novel molecular methods such as NGS in order to illuminate us about the genetical nature of medical conditions which in turns brings us and immense help for the management of so many diseases.
Because of the facilities and laboratory limitation that we are faced with now in Iran due to the sanctions, in this study we proceed to extract rna from the acetabular bone and cartilage of 40 adult patients undergoing total hip arthroplasty surgery with a simplified manual method of Guanidinium thiocyanate-phenol–chloroform (TRIzol) extraction.
Our overall results in the laboratory indicated an acceptable amount of rna residue. Through further investigation including 4 criteria of concentration, mass, RNA integrity number (RIN), and 28S/18S, we found out that 50 percent had suitable criteria in all field except the RIN.
With this new method an acceptable volume of rna with good mass, good 28s/18s and less pollution is easily derived from bone and cartilage but to access a better RIN further investigation is needed.
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| Keywords: RNA, manual extraction, cartilage, bone, ddh type 4 |
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Type of Study: Original Article |
Subject:
Genetics & Disease
Received: 2023/12/9 | Accepted: 2024/06/11 | Published: 2024/06/11
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