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Comparison of SARS-CoV-2 Antigen Detection by ELISA and Real-Time PCR in Colorectal Cancer Patients with Hypertension
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Ali Muhsen Ali *1   , Sharara Fadhil Abbood2 |
1- College of Applied Medical Science, University of Shatrah, Iraq , ali.muhsen@shu.edu.iq
2- College of Medicine, University of Kerbala, Iraq |
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Abstract: (254 Views) |
Colorectal cancer is a common form of cancer and tends to be associated with an increased viral infection severity, including after SARS-CoV-2 infection, in the presence of comorbidities such as hypertension. The presence of SARS-CoV-2 infection in patients with colorectal cancer can make their prognosis worse clinically, which requires the necessity of appropriate detection methods for early clinical intervention. Due to early diagnosis is one of the most important components of an outbreak control, a comparison study using PCR and ELISA to detect SARS-CoV-2 antigens in the patient population was carried out. Colorectal cancer and hypertensive patients were subjected to investigate the efficiency and reliability of both techniques. This case-control study comprised 30 cases of colorectal cancer patients with hypertension (HTN); 30 cases of HTN only; and 30 healthy controls without colorectal cancer or HTN. Mean age was 53.11±2.05 years. SARS-CoV-2 antigens in serum samples drawn from all groups were detected using enzyme-linked immunosorbent assay (ELISA) kits and real-time PCR. The detection frequency of SARS-CoV-2 antigens was higher in colorectal cancer patients with hypertension that were higher in the ELISA test known to be higher than in hypertension-only and healthy controls. In real-time PCR, the positive detection rate was 15% in CRC group and 8% in hypertension group, showing a higher sensitivity than ELISA only in CRC group (p < 0.05). Both methods were successful in detecting SARS-CoV-2, but real-time PCR presented a higher detection rate, indicating a higher viral load in the patients with colorectal cancer and with hypertension. ELISA results were confirmed by real-time PCR with higher sensitivity for detection of SARS-CoV-2 in real-time PCR. In this context, considering that ELISA is a low-cost tool, its application is suggested as an initial screening tool with positive samples for SARS-CoV-2 submitted to real-time PCR for confirmation. |
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| Keywords: SARS-CoV-2, Real-Time PCR, ELISA, Colorectal Cancer, Hypertension, Antigen Detection |
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Type of Study: Original Article |
Subject:
Other
Received: 2024/12/15 | Accepted: 2025/01/27 | Published: 2025/03/03
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