Grasses are natural homogenous group of plants and belong to the Gramineae family (Poaceae). The value of grasses to mankind has been recognized since the dawn of human civilization and culture of cereal grasses. In the present study, Leptochloa uniflora Hochst (Poaceae) was subjected to antioxidant and phytochemical profiles using UV-Vis, FTIR and HPTLC. Antioxidant assays were studied using DPPH, phosphomolybdenum and H2O2 scavenging activity. Antioxidant activities on L. uniflora showed efficient radical scavenging effects. Phytochemical profiles determined the presence of various bioactive compounds.  It is hoped that the present work revealed the utility of L. uniflora plant extracts for field applications.