:: Volume 4, Issue 1 (Int Biol Biomed J 2018) ::
IBBJ 2018, 4(1): 35-47 Back to browse issues page
Phytochemical Characterization of Marine Macro Alga Sargassum polycystem, Molecular Docking, and In Vitro Anti-bacterial Activity against Psuedomonas aeruginosa
Gopalan Rajkumar 1, Periyakali Saravana Bhavan * 2, Muthu Suganya 1, Veeran Srinivasan 3, Madhayan Karthik 1, Rajendran Udayasuriyan 1
1- Department of Zoology, Bharathiar University, Coimbatore, Tamil Nadu, India.
2- Department of Zoology, Bharathiar University, Coimbatore, Tamil Nadu, India. , bhavan@buc.edu.in
3- Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Abstract:   (1119 Views)
Marine macro algae are useful to mankind as food, biofertilizer, and source of bioactive compounds such as agar-agar, alginates, and carrageenan. The aim of this study was to explore the phytochemicals, and the antibacterial activity of the marine alga Sargassum polycystem. The marine alga was subjected to ethanolic and methanolic extractions. The preliminary screening for phytochemicals showed the presence of tannins, polyphenols, saponins, cardiac glycosides, and quinones. The GC-MS analysis revealed the presence of totally 16 secondary metabolites: 8 different compounds for each solvent extraction. Among these bioactive compounds, 3 compounds (1, 2 benzenedicarboxylic-dibutylester and 13, docosenamide of ethanolic extract, and 3, 5-diaminodeoxymethoxy of methanolic extract, showed the binding affinity and ability to react with exotoxin-A of Pseudomonas aeruginosa, a common pathogenic bacterium of fishes and prawns. The in vitro antibacterial assays revealed that both ethanolic and methanolic extracts of S. polycystem possessed the ability to inhibit the growth of P. aeruginosa. Therefore, aquaculture medicine could be prepared with S. polycystem.
Keywords: Brown alga, ligands, exotoxin-A, Sargassum polycystem
Full-Text [PDF 772 kb]   (320 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2017/09/30 | Accepted: 2017/10/29 | Published: 2017/12/1

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Volume 4, Issue 1 (Int Biol Biomed J 2018) Back to browse issues page