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The wound healing effect of fish scales isolated collagen sponge along with Eisenia foetida glycolipoprotein extract (G-90) in foot ulcer of diabetic rat
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Maryam M. Elmi1   , Fatemeh Elmi2 , Chiman Ghaderi3 , Farideh Feizi * 4 |
1- Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran
2- Department of Marine Chemistry, Faculty of Marine & oceanic sciences, University of Mazandaran, Babolsar, Iran
3- Department of Radiation Technology, Paramedical Faculty, Babol University of Medical Sciences, Babol, Iran
4- Department of Anatomical Science, and Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Science, Babol, Iran , feizi.dr@gmail.com |
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Abstract: (305 Views) |
Treatment of diabetic ulcers is associated with a failure in wound healing. While collagen dressing was applied effectively in wound healing, today, advanced dressing designs that can accelerate healing has received attention. Tissue homogenates glycolipoprotein (G-90) contains some growth factors that can participate in healing. The present study examined the wound healing effects of fish scale collagen sponge along with G-90 on sutured incisions in diabetic rats. G-90 was isolated from earthworm (Eisenia foetida). Type 1 collagen was purified from fish scales and lyophilized in sponge form. The sponges were immersed in 10 ng of G- 90. The efficacy of collagen sponge/G-90 was evaluated on sutured incisions in STZ-induced diabetic rats on days 4, 8, 14, and 18 through a morphological and histological approach. The results demonstrated that collagen sponge/G-90 is effective as a wound healing accelerator all days after wounding. Re-epithelialization and angiogenesis were significantly better in the treated group with collagen sponge/G-90 (P≤0.05). Experimental data demonstrated that collagen sponge/G-90 increased fibroblast proliferation, vascularization, collagen synthesis, and collagen fibril diameters. Considering the existence of many growth factors in G-90 and the role of collagen in the extracellular matrix, we conclude that the collagen sponge/G-90 could be a natural mixture for the management of wound healing. Additional studies are proposed to approve it as a wound-healing agent. |
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| Keywords: collagen sponge, G-90, diabetic rat, earthworm, wound healing |
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Full-Text [PDF 787 kb]
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Type of Study: Original Article |
Subject:
Other
Received: 2022/07/3 | Accepted: 2023/01/27 | Published: 2024/02/29
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