Polymorphism and Genetic Diversity of BAT25 Marker in Colorectal Cancer
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Arame Ndiaye * 1, Fatimata Mbaye2 , Bineta Kénémé2 , Fatou Diallo2 , Abdourahmane Samba2 , Fatou Cisse2 , Souleymane Thiam2 , Dominique Doupa3 , Papa saloum Diop4 , Mbacke Sembene2 , Niama Diop Sall5 |
1- Laboratory of Molecular Biochemistry –Biology, Cheikh Anta Diop Universityof Dakar, Senegal. , yssndiay@yahoo.fr 2- Department of Animal Biology, Faculty of Science, Cheikh Anta Diop University of Dakar, Senegal. 3- Laboratory of Molecular Biochemistry-Biology, Training and Research Unit, Faculty of Medicine (UFR), Gaston Berger University, Saint Louis, Senegal. 4- Department of digestive surgery and hepatobiliary-Cancerology, Grand Yoff Hospital, Dakar, Senegal. 5- Laboratory of Molecular Biochemistry –Biology, Cheikh Anta Diop Universityof Dakar, Senegal. |
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Abstract: (7639 Views) |
By its frequency, colorectal cancer (CRC) has become a serious public health problem. BAT25 marker is included in the panel of five markers dedicated to the diagnosis of CCR microsatellite instability. The objective of this study was to evaluate the polymorphism and genetic diversity of the BAT25 marker in CRC cases in Senegal. This prospective study was performed on 22 CRC patients. After DNA extraction, the polymorphism and genetic diversity of BAT25 was determined by PCR and sequencing. The alignment of the sequences was carried out using Bio Edit software. The parameters of the polymorphism and genetic variability were determined using the Dnasp, Mega and the Arelquin programs. The results showed a genetic variability of BAT25. This variability is represented by mutations observed in the tumor tissues. The most frequent mutation was the deletion of a thymine at position 72 (T72d). This deletion was absent in healthy tissues and controls. From this study, it can be concluded that the mutations found in the tumor tissues could have a role in the onset, development and progression of CRC. |
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Keywords: Colorectal cancer, microsatellite, BAT25, polymorphism |
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Type of Study: Case Series |
Subject:
Cancer Received: 2017/05/2 | Accepted: 2017/06/26 | Published: 2017/07/30
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